Proteinase K Solution（20 mg/mL, RNase-Free, DNase-Free）_ 10412ES Interferon the protein migrates to 50-60

the protein migrates to 50-60 kDa based on Tris-Bis PAGE result

1× IVT buffer

MG132 is a potent

[3] Song M

Adding samples and preparing solutions is best done on ice

Proteinase K Solution（20 mg/mL, RNase-Free, DNase-Free）_ 10412ES Interferon the protein migrates to 50-60Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids with a relative molecular weight of 29. 3 kDa. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide . Proteinase K is widely used for the preparation of chromosomal DNA , and removal of DNases and RNases when isolating DNA and RNA from tissues or cell lines. Denaturants such as SDS (1%) can